Agilent Technologies Switch 5185 5991 User Manual

5185-5991 5 KDa MWCO 4 mL  
5188-5201 30 KDa MWCO 4 mL  
5188-5202 50 KDa MWCO 4 mL  
5188-2798 5 KDa MWCO 15 mL  
5188-2799 30 KDa MWCO 15 mL  
5188-2800 50 KDa MWCO 15 mL  
Agilent Spin Concentrators for Proteins  
Instructions – 4 mL Concentrators  
Equipment required  
Centrifuge  
4 mL Spin Concentrators, pack of 25  
Rotor type  
Swing bucket or Fixed angle  
Minimum rotor angle  
Rotor cavity  
25°  
To fit 15 mL (17 mm) conical bottom tubes  
Minimum effective speed  
Recommended speed  
Maximum speed  
Concentrate recovery  
Pipette type  
Recommended tip  
Technical specifications  
Concentrator sample volume capacity  
Swing bucket rotor  
Fixed angle rotor  
Dimensions  
2,000 g  
5,000 g  
7,500 g  
Fixed or variable volume  
Thin gel loader type  
1-4 mL  
1-4 mL  
Total Length  
Width  
Active Membrane Area  
Hold-up volume of membrane  
Dead stop volume  
pH range  
122 mm  
17 mm  
2.0 cm2  
<10 µL  
20 µL  
5,000 Molecular Weight Cut-Off (MWCO)  
4 mL Spin Concentrator, Part No. 5185-5991  
pH 1 – 9  
Materials of construction  
Body  
Filtrate vessel  
Concentrator cap  
Membrane  
Polycarbonate  
Polypropylene  
Polycarbonate  
Polyethersulfone  
Concentrating Proteins with 4 mL Concentrators:  
Desalting/Buffer Exchange  
1
2
3
Fill concentrator with sample within the working volume  
capacity range noted in the table above (ensure that the hinged  
lid is fully seated).  
1
Concentrate sample to desired level or at least  
5P.  
2
3
4
Empty filtrate container.  
Insert assembled concentrator into centrifuge (when fixed  
angle rotors are used, angle the concentrators so that the  
printed windows face upwards/outwards).  
Centrifuge at recommended speeds, taking care not to exceed  
the maximum g force indicated in the table above (20 minutes  
at 7,500 g is recommended for 30P concentration of 4 mL at  
10LC).  
Refill concentrator with an appropriate buffer.  
Concentrate the sample again and repeat the  
process until the concentration of the  
contaminating species is sufficiently reduced.  
Typically three wash cycles will remove 99% of  
initial salt content.  
4
Once the desired concentration is achieved, remove assembly  
and recover sample from the bottom of the concentrate pocket  
with a pipette.  
See Notes on next page.  
 

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